A ligand-mediated nanovector for targeted gene delivery and transfection in cancer cells

A visible, targeted high-efficiency gene delivery and transfection strategy

BACKGROUND To enhance myocardial angiogenic gene expression, a novel gene delivery strategy was tested. Direct intramyocardial injection of an angiogenic gene with microbubbles and insonation were applied in a dog animal model. Dogs received one of the four different treatments in conjunction with either the enhanced green fluorescence protein (EGFP) gene or the hepatocyte growth factor (HGF) g...

Poly(ethylenimine)-mediated transfection: a new paradigm for gene delivery.

Poly(ethylenimine) (PEI) is a synthetic polycation that has been used successfully for gene delivery both in vitro and in vivo due, in theory, to a form of protection that is afforded to the carried plasmids. In this study the stability of PEI/DNA complexes was demonstrated using deoxyribonuclease (DNase) 1 and DNase 2, various levels of pH, and increasing exposure times. DNA that was complexed...

Epidermal Growth Factor Receptor-Targeted Engineered Gelatin Nanovectors for Gene Delivery and Transfection in Pancreatic Cancer Cells

Pancreatic cancer is a leading cause of cancerdeath, mostly owing to lack of efficient therapeutic options. Upwards of 90% pancreatic tumors over express epidermal growth factor receptor (EGFR) and this largely influences the disease aggressiveness and poor clinical outcome in patients. Non-viral gene therapy has achieved increased attention over the last two decades. In this study type B gelat...

design and development of a series of recombinant fusion vectors for targeted cancer gene delivery

Therapeutic Gene Delivery and Transfection in Human Pancreatic Cancer Cells using Epidermal Growth Factor Receptor-targeted Gelatin Nanoparticles

More than 32,000 patients are diagnosed with pancreatic cancer in the United States per year and the disease is associated with very high mortality (1). Urgent need exists to develop novel clinically-translatable therapeutic strategies that can improve on the dismal survival statistics of pancreatic cancer patients. Although gene therapy in cancer has shown a tremendous promise, the major chall...